| Registro completo |
| Provedor de dados: |
Trop. Plant Pathol.
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| País: |
Brazil
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| Título: |
Analysis of the nucleotide sequence of the coat protein and 3'-untranslated region of two Brazilian Potato virus Y isolates
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| Autores: |
INOUE-NAGATA,ALICE K.
FONSECA,M. ESTHER N.
LOBO,TATIANA O.T.A.
ÁVILA,ANTÔNIO C. DE
MONTE,DAMARES C.
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| Data: |
2001-03-01
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| Ano: |
2001
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| Palavras-chave: |
PVY
Potyvirus
Phylogenetic analysis
Taxonomy
Coat protein
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| Resumo: |
Two Brazilian Potato virus Y (PVY) isolates were biologically characterized as necrotic (PVY-NBR) and common (PVY-OBR) based upon symptoms on test plants. Additional characterization was performed by sequencing a cDNA corresponding to the 3' terminal region of the viral genome. The sequence consisted of 195 nucleotides (nt) coding part of the nuclear inclusion body b (NIb) gene, 804 nt of the coat protein (CP) gene, and 328 nt (PVY-OBR) or 326 nt (PVY-NBR) of the 3'-untranslated region (UTR). Translation of the sequence resulted in one single open reading frame with part of the NIb and a CP of 267 amino acids. The two isolates shared 95.1% similarity in the CP amino acid sequence. The CP and the 3'-UTR sequence of the Brazilian isolates were compared to those of other PVY isolates previously reported and unrooted phylogenetic trees were constructed. The trees revealed a separation of two distinct clusters, one comprising most of the common strains and the other comprising the necrotic strains. PVY-OBR was clustered in the common group and PVY-NBR in the necrotic one.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000100008
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| Editor: |
Sociedade Brasileira de Fitopatologia
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| Relação: |
10.1590/S0100-41582001000100008
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| Formato: |
text/html
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| Fonte: |
Fitopatologia Brasileira v.26 n.1 2001
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| Direitos: |
info:eu-repo/semantics/openAccess
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